During the experiment, hydrogen peroxide was applied in the nutrient solution at concentrations of 25 ppm, 50 ppm, and 75 ppm active substance. The application aims to disinfect water and prevent biofilm buildup in irrigation systems. This trial was designed to ensure that disinfection residues have no negative effects on roots. Throughout the trial period, the plants were carefully and systematically monitored to detect any potential effects on crop development, plant growth, and root health. As the roots were in direct contact with the nutrient solution, particular attention was given to identifying any signs of root damage or root burn.

The roots were therefore regularly evaluated based on their development and appearance. Root quantity was assessed through the visible root formation at the bottom of the plant block, while root colour was used as an indicator of plant health. White roots reflected a healthy condition without signs of stress or burn damage, and the presence of brown discolouration indicated potential root damage.